The spotlight was on UCLA Chancellor Gene Block, whose university saw large protests and a camp that was attacked by counter-protesters.
Police and school officials were slow to respond to violence and were heavily criticised for their response last month.
On Wednesday, the police chief at UCLA was temporarily removed and reassigned “pending an examination of our security processes”, the university said.
In his opening remarks, Mr Block said the protesters had “tested the limits” of the UCLA administration’s de-escalation strategy. He said his university strove to prevent using law enforcement to make arrests on campus “unless it is absolutely necessary to protect the physical safety of the community”.
Congresswoman Ilhan Omar, a Democrat, accused Mr Block of not protecting pro-Palestinian students by allowing counter-protesters to attack for hours.
Meanwhile, photos and videos showed a new small camp being built outside Kerckhoff Hall at UCLA on Thursday.
Protesters used tables, metal fences, and wooden boards to block off walkways, while security guards watched over them, according to CBS, the BBC’s US partner.
UCLA Vice Chancellor for Strategic Communications told BBC News that the administration was “aware of the demonstration activity on the Kerckhoff patio”.
“Our safety personnel are on site and actively monitoring the situation.”
A later statement issued by Mr Block and the UCLA vice chancellor of campus safety said the administration “has withdrawn consent to remain on campus for the demonstrators,” and is “asking them to disperse immediately”.
“There is reasonable cause to find that demonstrators’ activities — including erecting barricades, establishing fortifications, and blocking access to parts of the campus and buildings — are disrupting campus operations,” the statement continued.
“Demonstrators have been informed that if they do not disperse, they will face arrest and possible disciplinary action.”
Some classes switched to remote learning due to the new protest on Thursday, and Los Angeles police issued a citywide tactical alert, according to KTLA-TV.
The alerts are used to re-distribute on-duty police officers and prepare for a possible escalation that would trigger an emergency.
At Harvard University on Thursday, over 1,000 graduating students stood up and left their commencement ceremony in protest after the administration barred 13 students from graduating due to their protest activities.
Members of the audience also booed the university president as he spoke, and Palestinian flags were seen flying near the back of the crowd.
Previous hearings by the House of Representatives committee have resulted in two Ivy League university heads stepping down, though Thursday’s hearing had tense moments it was much less contentious than the previous committee hearings.
The appearance of Columbia University’s president Dr Nemat Shafik at an April congressional hearing emboldened students on campus and led to them pitching tents on a lawn, sparking a wave of protests across the US.